"RNA and genome stability” funded by MIUR (PRIN 2017) : Transcription can be a source of genome instability, especially through the formation of DNA:RNA hybrids intermediates. Importantly, RNA and its potential to form a hybrid with single-stranded DNA is now emerging as key positive regulator of the pathways maintaining genome stability too. Therefore, the study of the emerging role of RNA both as threat and a guardian of genome stability is extremely important. Our aim is to investigate the mechanisms leading to DNA:RNA hybrid formation and those preventing their formation, under physiological and pathological conditions, using the yeast Saccharomyces cerevisiae and mammalian cells as model systems.
Bonetti D, Rinaldi C, Vertemara J, Notaro M, Pizzul P, Tisi R, Zampella G, Longhese MP (2019). DNA binding modes influence Rap1 activity in the regulation of telomere length and MRX functions at DNA ends. Nucleic Acids Res doi: 10.1093/nar/gkz1203
Villa M, Bonetti D, Carraro M, Longhese MP (2018) Rad9/53BP1 protects stalled replication forks from degradation in Mec1/ATR defective cells. EMBO Rep. 19: 351-367
Graf M, Bonetti D, Lockhart A, Serhal K, Kellner V, Maicher A, Jolivet P, Teixeira MT, Luke B (2017). Telomere Length Determines TERRA and R-Loop Regulation through the Cell Cycle. CELL Jun 29;170(1):72-85.e14. doi: 10.1016/j.cell.2017.06.006
Bonetti D, Villa M, Gobbini E, Cassani C, Tedeschi G, Longhese MP (2015). Escape of Sgs1 from Rad9 inhibition reduces the requirement for Sae2 and functional MRX in DNA end resection. EMBO Reports Mar;16(3):351-61. doi: 10.15252/embr.201439764