Bonetti Diego, PhD

Assistant Professor in Genetics
Bonetti Diego BtBs UNIMIB

 

room 5050, building U3, tel. +39 02 6448 3545

lab 5052, building U3, tel. +39 02 6448 3549

diego.bonetti1@unimib.it

see also: PudMed, Linkedin, ResearchGate

The activity is focused on the following research lines
Health research area BtBs UNIMIB
Health

Aging Diseases, Cancer, Neurodegenerative and neurological diseases

Keywords
genome stability, genetics, S. cerevisiae, DNA damage, telomeres, R loops

Research areas

Maintenance of genome stability in eukaryotes: DNA damage checkpoints, DNA repair, telomeres and RNA-DNA hybrids as a threat and a guardian of genome stability

Research projects

"RNA and genome stability” funded by MIUR (PRIN 2017) : Transcription can be a source of genome instability, especially through the formation of DNA:RNA hybrids intermediates. Importantly, RNA and its potential to form a hybrid with single-stranded DNA is now emerging as key positive regulator of the pathways maintaining genome stability too. Therefore, the study of the emerging role of RNA both as threat and a guardian of genome stability is extremely important. Our aim is to investigate the mechanisms leading to DNA:RNA hybrid formation and those preventing their formation, under physiological and pathological conditions, using the yeast Saccharomyces cerevisiae and mammalian cells as model systems.

Selected articles

Bonetti D, Rinaldi C, Vertemara J, Notaro M, Pizzul P, Tisi R, Zampella G, Longhese MP (2019). DNA binding modes influence Rap1 activity in the regulation of telomere length and MRX functions at DNA ends. Nucleic Acids Res doi: 10.1093/nar/gkz1203

Villa M, Bonetti D, Carraro M, Longhese MP (2018) Rad9/53BP1 protects stalled replication forks from degradation in Mec1/ATR defective cells. EMBO Rep. 19: 351-367

Graf M, Bonetti D, Lockhart A, Serhal K, Kellner V, Maicher A, Jolivet P, Teixeira MT, Luke B (2017). Telomere Length Determines TERRA and R-Loop Regulation through the Cell Cycle. CELL Jun 29;170(1):72-85.e14. doi: 10.1016/j.cell.2017.06.006

Bonetti D, Villa M, Gobbini E, Cassani C, Tedeschi G, Longhese MP (2015). Escape of Sgs1 from Rad9 inhibition reduces the requirement for Sae2 and functional MRX in DNA end resection. EMBO Reports Mar;16(3):351-61. doi: 10.15252/embr.201439764

International and national collaborations

Maria Pia Longhese, UNIMIB

Michela Clerici, UNIMIB

Marco Foiani, IFOM (FIRC Institute of Molecular Oncology)

Fabrizio D’Adda di Fagagna, IFOM (FIRC Institute of Molecular Oncology)

Brian Luke, IMB Mainz


Bonetti’s Lab – #BonettiLab_BtBs
last update: September 2020