Department of Biotechnology and Biosciences BtBs
Natalello Antonino, PhD
Associate Professor, Applied Physics
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room 4050, building U3, tel. +39 02 6448 3459
lab 4051, building U3, tel. +39 02 6448 3461
see also (inserire l’url o ID): PudMed, ResearchGate, Google Scholar, Scopus, ORCID
Graphical abstract
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Keywords
Amyloids, Bioanalytical Methods, Biophysics, FTIR Micro-spectroscopy, FTIR spectroscopy, Intact-cell Spectroscopy, Lipids, Protein Aggregation, Protein Stability, Spectroscopy.
Research Group
Ami Diletta – PostDoc Fellow
room 4051/4050, IV floor, building U3, tel: +39 02 6448 3461/3459, diletta.ami@unimib.it
Lopez Arriz Martin Antonio – Master student
room 4051, IV floor, building U3, tel: +39 02 6448 3461, m.lopezarriz@campus.unimib.it
Research interest
-Protein folding, misfolding and aggregation in vitro and in situ studied by biophysical approaches, including infrared spectroscopy and micro-spectroscopy, optical spectroscopies (absorption, florescence, circular dichroism), and mass spectrometry. Major research topics: 1) protein stability; 2) amyloid aggregation; 3) structure-function relation; 4) aggregation of recombinant proteins; 5) protein-protein and protein-ligand interactions; 6) nano- and bio-materials.
-Bioanalytical applications of spectroscopic methods. Major research topics: FTIR (micro)spectroscopy of complex biological systems; in situ studies of biological processes; spectroscopic markers of neurodegenerative disorders in biofluids.
Selected publications
-Sala BM, Le Marchand T, Pintacuda G, Camilloni C, Natalello A, Ricagno S. Conformational Stability and Dynamics in Crystals Recapitulate Protein Behavior in Solution. Biophys J. 2020; 119(5):978-988. doi: 10.1016/j.bpj.2020.07.015.
-Ami D, Mereghetti P, Foli A, Tasaki M, Milani P, Nuvolone M, Palladini G, Merlini G, Lavatelli F, Natalello A. ATR-FTIR Spectroscopy Supported by Multivariate Analysis for the Characterization of Adipose Tissue Aspirates from Patients Affected by Systemic Amyloidosis. Anal Chem. 2019; 91(4):2894-2900. doi: 10.1021/acs.analchem.8b05008.
-Ami D, Lavatelli F, Rognoni P, Palladini G, Raimondi S, Giorgetti S, Monti L, Doglia SM, Natalello A, Merlini G. In situ characterization of protein aggregates in human tissues affected by light chain amyloidosis: a FTIR microspectroscopy study. Sci Rep. 2016; 6:29096. doi: 10.1038/srep29096.
-Natalello A, Mangione PP, Giorgetti S, Porcari R, Marchese L, Zorzoli I, Relini A, Ami D, Faravelli G, Valli M, Stoppini M, Doglia SM, Bellotti V, Raimondi S. Co-fibrillogenesis of Wild-type and D76N β2-Microglobulin: THE CRUCIAL ROLE OF FIBRILLAR SEEDS. J Biol Chem. 2016;291(18):9678-89. doi: 10.1074/jbc.M116.720573.
Natalello’s Lab – #NatalelloLab_BtBs
last update: May 2021
BtBs - Google Calendar
BtBs - Google Calendar for seminars, events, congresses, meetings, etc. of the Department of Biotechnology and Biosciences (BtBs) - UNIMIB
To add this calendar to your Google Calendar:
1-login on the Google Calendar to which you would like to associate this calendar.
2-click on the following link
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4-find this calendar under "other calendars".
NB: at any time you can delete it or not display it by removing the tick on the calendar.
EXTERNAL - Google calendar for seminars, events, congresses, etc. outside our department (BtBs) but that deal with our research topics.
To add this calendar to your Google Calendar:
1-login on the Google Calendar to which you would like to associate this calendar.
2-click on the following link
3-click "add" on "add calendar" request
4-find this calendar under "other calendars".
NB: at any time you can delete it or not display it by removing the tick on the calendar.
BtBs Seminars 2024 calendar
seminar list and mailing list subscription
Ferrari Daniela, PhD
Assistant Professor in Applied Biology
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room 3052, building U3, tel. +39 02 6448 3368
lab 3013/2014, building U3, tel. +39 02 6448 3365/3366
Ferrari’s Lab – #FerrariLab_BtBs
last update: September 2020
Research area: BIOMEDICINE
Immagine Slide
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Sottotitolo
the research groups involved
Peso
-3
Metabolic characterization of maturing human pluripotent stem cell-derived cardiomyocytes
Giulia Emanuelli – Cardiovascular School, King’s College, London
Zampella Giuseppe
Associate Professor of General and Inorganic Chemistry
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Research projects
-ZAMPELLA-Fondo per il finanziamento delle attività base di ricerca
Anno: 2017
Bando: FFABR 2017
Enti finanziatori: M.I.U.R. - MINISTERO DELL'ISTRUZIONE, DELL'UNIVERSITA' E DELLA RICERCA - UFFICIO I - Bilancio e Contabilita'. Coordinamento staff della Direzione
Publications
Panzeri, D., Toini, E., Vertemara, J., Silvestri, G., Bunea, V., Zecca, G., et al. (2024). Small proteins, great promises: geographic bioprospecting of Bowman-Birk protease Inhibitors and domestication side-effects in African cowpea (Vigna unguiculata L.). PLANTS, PEOPLE, PLANET, 6(4 (July 2024)), 921-934 [10.1002/ppp3.10507].
Ami, D., Santambrogio, C., Vertemara, J., Bovio, F., Santisteban-Veiga, A., Sabín, J., et al. (2024). The Landscape of Osteocalcin Proteoforms Reveals Distinct Structural and Functional Roles of Its Carboxylation Sites. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 146(40), 27755-27769 [10.1021/jacs.4c09732].
Silvestri, G., Arrigoni, F., Persico, F., Bertini, L., Zampella, G., De Gioia, L., et al. (2023). Assessing the Performance of Non-Equilibrium Thermodynamic Integration in Flavodoxin Redox Potential Estimation. MOLECULES, 28(16) [10.3390/molecules28166016].
Arrigoni, F., De Gioia, L., Elleouet, C., Pétillon, F., Schollhammer, P., Talarmin, J., et al. (2023). Normal vs. Inverted Ordering of Reduction Potentials in [FeFe]-Hydrogenases Biomimetics: Effect of the Dithiolate Bulk. CHEMISTRY-A EUROPEAN JOURNAL, 29(38 (July 6, 2023)), 1-12 [10.1002/chem.202300569].
Zampella’s Lab – #ZampellaLab_BtBs
last update: April 2025
Tisi Renata Anita, PhD
Associate Professor in Molecular Biology
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room 5017, building U4, tel. +39 02 6448 3522
lab 5044, building U4, tel. +39 02 6448 3534
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Research group
Vertemara Jacopo – PostDoc
room 4064, building U3, tel. +39 02 6448 3473, jacopo.vertemara@unimib.it
Keywords
Homology modelling; Small molecule-protein docking; Protein-protein docking
Research areas
-Signal transduction
-Computational Biology
-Systems Biology
-Biological systems analysis, modelling and simulation
Selected publications
-Marsella A, Gobbini E, Cassani C, Tisi R, Cannavo E, Reginato G, Cejka P, Longhese MP. Sae2 and Rif2 regulate MRX endonuclease activity at DNA double-strand breaks in opposite manners. Cell Rep. 2021 Mar 30;34(13):108906. doi: 10.1016/j.celrep.2021.108906.
-Tisi R, Spinelli M, Palmioli A, Airoldi C, Cazzaniga P, Besozzi D, Nobile MS, Mazzoleni E, Arnhold S, De Gioia L, Grandori R, Peri F, Vanoni M, Sacco E. The Multi-Level Mechanism of Action of a Pan-Ras Inhibitor Explains its Antiproliferative Activity on Cetuximab-Resistant Cancer Cells. Front Mol Biosci. 2021 Feb 17;8:625979. doi: 10.3389/fmolb.2021.625979.
-Tisi R, Gaponenko V, Vanoni M, Sacco E. Natural Products Attenuating Biosynthesis, Processing, and Activity of Ras Oncoproteins: State of the Art and Future Perspectives. Biomolecules. 2020 Nov 10;10(11):1535. doi: 10.3390/biom10111535.
-Tisi R, Vertemara J, Zampella G, Longhese MP. Functional and structural insights into the MRX/MRN complex, a key player in recognition and repair of DNA double-strand breaks. Comput Struct Biotechnol J. 2020 May 16;18:1137-1152. doi: 10.1016/j.csbj.2020.05.013.
Tisi’s Lab – #TisiLab_BtBs
last update May 2021