Dipartimento di Biotecnologie e Bioscienze BtBs
Santambrogio Carlo, PhD
Assistant Professor of General Biochemistry
Image
room 5065, building U3, tel. +39 02 6448 3363
lab 2i42, building U3, tel. +39 02 6448 3392
Keywords
Structural biology; Protein conformational ensemble; Protein-protein & protein-ligand interaction; Intrinsically disordered proteins; Amyloid aggregation; Proteomics; Mass spectrometry; Biochemical and biophysical methods
Research areas
Nanomedicine and biomaterials; Neurodegenerative and neurological diseases; Molecular modeling and structural biology
Santambrogio’s Lab - #SantambrogioLab_BtBs
last update March 2020
CANCELLED - mini-symposium: Structural biology for human diseases
Friday, March 27th, 2020, 14:30-16:30, room U4-04, building U4, UNIMIB
CANCELLED - 3rd Joint Workshop of the SIB groups
27th February 2020
Room U4-08 Luisella Sironi, UNIMIB, Piazza della Scienza 4, Milan.
Natalello Antonino, PhD
Associate Professor, Applied Physics
Image
room 4050, building U3, tel. +39 02 6448 3459
lab 4051, building U3, tel. +39 02 6448 3461
see also (inserire l’url o ID): PudMed, ResearchGate, Google Scholar, Scopus, ORCID
Graphical abstract
Image
Keywords
Amyloids, Bioanalytical Methods, Biophysics, FTIR Micro-spectroscopy, FTIR spectroscopy, Intact-cell Spectroscopy, Lipids, Protein Aggregation, Protein Stability, Spectroscopy.
Research Group
Ami Diletta – PostDoc Fellow
room 4051/4050, IV floor, building U3, tel: +39 02 6448 3461/3459, diletta.ami@unimib.it
Lopez Arriz Martin Antonio – Master student
room 4051, IV floor, building U3, tel: +39 02 6448 3461, m.lopezarriz@campus.unimib.it
Research interest
-Protein folding, misfolding and aggregation in vitro and in situ studied by biophysical approaches, including infrared spectroscopy and micro-spectroscopy, optical spectroscopies (absorption, florescence, circular dichroism), and mass spectrometry. Major research topics: 1) protein stability; 2) amyloid aggregation; 3) structure-function relation; 4) aggregation of recombinant proteins; 5) protein-protein and protein-ligand interactions; 6) nano- and bio-materials.
-Bioanalytical applications of spectroscopic methods. Major research topics: FTIR (micro)spectroscopy of complex biological systems; in situ studies of biological processes; spectroscopic markers of neurodegenerative disorders in biofluids.
Selected publications
-Sala BM, Le Marchand T, Pintacuda G, Camilloni C, Natalello A, Ricagno S. Conformational Stability and Dynamics in Crystals Recapitulate Protein Behavior in Solution. Biophys J. 2020; 119(5):978-988. doi: 10.1016/j.bpj.2020.07.015.
-Ami D, Mereghetti P, Foli A, Tasaki M, Milani P, Nuvolone M, Palladini G, Merlini G, Lavatelli F, Natalello A. ATR-FTIR Spectroscopy Supported by Multivariate Analysis for the Characterization of Adipose Tissue Aspirates from Patients Affected by Systemic Amyloidosis. Anal Chem. 2019; 91(4):2894-2900. doi: 10.1021/acs.analchem.8b05008.
-Ami D, Lavatelli F, Rognoni P, Palladini G, Raimondi S, Giorgetti S, Monti L, Doglia SM, Natalello A, Merlini G. In situ characterization of protein aggregates in human tissues affected by light chain amyloidosis: a FTIR microspectroscopy study. Sci Rep. 2016; 6:29096. doi: 10.1038/srep29096.
-Natalello A, Mangione PP, Giorgetti S, Porcari R, Marchese L, Zorzoli I, Relini A, Ami D, Faravelli G, Valli M, Stoppini M, Doglia SM, Bellotti V, Raimondi S. Co-fibrillogenesis of Wild-type and D76N β2-Microglobulin: THE CRUCIAL ROLE OF FIBRILLAR SEEDS. J Biol Chem. 2016;291(18):9678-89. doi: 10.1074/jbc.M116.720573.
Natalello’s Lab – #NatalelloLab_BtBs
last update: May 2021