Seminario - Biotecnologie e Bioscienze - Giovedì 10 aprile 2025, ore 16:30, edificio BIOS-U3, aula U3-04
Miriana Dardano, Hannover Medical School, Germany
Abstract
Human pluripotent stem cells (hPSCs) are used to generate a broad range of organoids; self-organized, complex 3D structures resembling aspects of the architecture, cell composition and function of tissues found in embryogenesis or adult organs. By modulating our hPSC-based heart-forming organoid (HFO) model 1 via stage-specific supplementation of hemato-endothelial factors, we have recently established the novel, so-called blood-generating (BG)-HFO 2. BG-HFOs comprise functional cardiac tissue with a ventricular-like phenotype, while featuring distinct endothelial subtypes, including a mesenchyme-embedded endothelial layer that generates hematopoietic cells possessing erythroid, myeloid and lymphoid potential. The morphological structure of BG-HFOs reflects aspects of cardiogenesis and hematopoiesis in vivo. BG-HFOs provide a platform for pharmacological testing, disease modeling and developmental studies, overcoming the limitations of studying these processes in human embryos.
1.Drakhlis, L. et al. Human heart-forming organoids recapitulate early heart and foregut development. Nature Biotechnology 39, 737-746 (2021).
2.Dardano, M. et al. Blood-generating heart-forming organoids recapitulate co-development of the human haematopoietic system and the embryonic heart. Nature Cell Biology (2024).
Ospite: Antonella Ronchi
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