Spotlight on PhD Students: “Resection of a DNA Double-Strand Break by Alkaline Gel Electrophoresis and Southern Blotting”

Mercoledì 23 Giugno 2021
Book section wrote by Erika Casari and colleagues on Homologous Recombination, published in 2021
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TeCSBi SPOTLIGHT on PhD students

Comment by Valentina Artusa

Methods in Molecular Biology is a book series that introduces the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice. Published every year, the series provides updated, tested and trusted, comprehensive and reliable protocols, indexed in PubMed. Our TeCSBi PhD Student, Erika Casari (Longhese’s Lab), contributed to this year's edition of the Homologous Recombination book with a section focused on describing a Southern blot-based method to visualize the generation of ssDNA at the ends of site-specific DSBs generated in the Saccharomyces cerevisiae genome. Erika, who’s scholarship is funded by the Department within “Progetto di Eccellenza del Dipartimento di Biotecnologie e Bioscienze ‘CHRonical multifactorial disorders explored by NOvel integrated Strategies (CHRONOS)”, is pursuing her PhD entitled: “Synthetic cytotoxicity to target DNA repair in cancer”.

Big congratulations to Erika for this achievement!

Find out more using the following DOI: 10.1007/978-1-0716-0644-5_3

Hashtags:  #BtBsPhD #TeCSBi  #TeCSBispotlight #LongheseLab_BtBs #CHRONOS_BtBs