A mass spectrometry facility for the analysis of small and large molecules from pure samples or complex matrices for quantitation, structural characterization and omics investigation by ultra-high resolution data.
Instrumentation (Thermo Fisher Scientific): Orbitrap Fusion™ Tribrid™ coupled to EASY-nLC 1000 UHPLC, TSQ Quantum™ Access MAX coupled to UltiMate 3000 UHPLC.
Head of the Mass spectrometry facility: Prof. Rita Grandori
Web page: Coming soon!
The facility offers analysis of small and large molecules from pure samples or complex matrices for structural characterization or omics investigation. The laboratory is equipped with two instruments from Thermo Fisher Scientific, an Orbitrap Fusion and a TSQ Quantum Access MAX.
The Orbitrap Fusion mass spectrometer enables the analysis of molecules and supramolecular complexes in the 50-6,000 m/z range, with high resolution (up to R=450,000), scan rate (MSn up to 20 Hz), mass accuracy (< 3 ppm), dynamic range (> 5,000) and sensitivity (100 fg total amount of the reserpine standard). The instrument is characterized by a great flexibility of scan protocols based on multiple fragmentation modes, i.e. collision-induced dissociation (CID), higher-energy collisional dissociation (HCD), electron-transfer dissociation (ETD) and their combinations, that can be performed in two different collision cells (quadrupole or linear ion trap) and analyzed by two different analyzers (linear ion trap or Orbitrap). These features support bottom-up, top-down and middle-down proteomics studies and enable high-throughput and high-depth analysis of small molecules, peptides, proteins, post-translational modifications, protein-ligand interactions, and other polymers. Quantitative shotgun proteomics can be implemented by either stable-isotope or label-free approaches. The instrument is equipped with a regular and a nano electrospray ionization (ESI) sample source and can be coupled to a nano-flow UHPLC EASY-nLC 1000, enabling automated, high-performance LC/MS analyses of complex biological matrices. Proteomics data analysis is performed by the software Proteome Discoverer 2.2.
In addition, we offer special analysis packages at a reduced price, such as intact protein exact mass determination, phosphorylation characterization, SDS-PAGE band identification, label-based or label-free protein quantitation.
The TSQ Quantum Access MAX instrument is a Triple Quadrupole analyzer equipped with a HESI ionization probe and an 90° high-efﬁciency square quadrupole collision cell. The instrument enables the analysis of molecules in the mass range of 10–3,000 m/z at 5,000 µ/s scan rate and with 25 ms positive/negative switching. Scan functions include full scan MS in Q1 or Q3, selected ion monitoring (SRM) in Q1 or Q3, selected reaction monitoring (SRM), product ion scanning, precursor ion scanning and neutral loss scanning. In SRM it is possible to define up to 3,000 timed segments and 2 ms dwell times. These features support targeted analysis of hundreds of compounds in a single run. In addition, the quantitation-enhanced data-dependent MS/MS (QED-MS/MS) provides simultaneous compound confirmation and quantification. Applications of small molecules quantitation cover the environmental, food, pharma and clinical research. The mass spectrometer is coupled with a UltiMate LPG-3400SD Gradient Pump that supports 4 eluents via a proportioning valve with a settable flow of 0.001 to 10.000 mL/min at pressures up to 620 bar. This LC system support standard LC applications as well offers full compatibility with fast UHPLC separations. Data acquisition and analysis is performed by the software Xcalibur.