Seminar - Biotechnology and Biosciences - Thursday 04/10/2025, 4:30 pm, U3-BIOS building, room U3-04
Miriana Dardano, Hannover Medical School, Germany
Abstract
Human pluripotent stem cells (hPSCs) are used to generate a broad range of organoids; self-organized, complex 3D structures resembling aspects of the architecture, cell composition and function of tissues found in embryogenesis or adult organs. By modulating our hPSC-based heart-forming organoid (HFO) model 1 via stage-specific supplementation of hemato-endothelial factors, we have recently established the novel, so-called blood-generating (BG)-HFO 2. BG-HFOs comprise functional cardiac tissue with a ventricular-like phenotype, while featuring distinct endothelial subtypes, including a mesenchyme-embedded endothelial layer that generates hematopoietic cells possessing erythroid, myeloid and lymphoid potential. The morphological structure of BG-HFOs reflects aspects of cardiogenesis and hematopoiesis in vivo. BG-HFOs provide a platform for pharmacological testing, disease modeling and developmental studies, overcoming the limitations of studying these processes in human embryos.
1.Drakhlis, L. et al. Human heart-forming organoids recapitulate early heart and foregut development. Nature Biotechnology 39, 737-746 (2021).
2.Dardano, M. et al. Blood-generating heart-forming organoids recapitulate co-development of the human haematopoietic system and the embryonic heart. Nature Cell Biology (2024).
Host: Antonella Ronchi
The seminar is OPEN to all.
ONLINE REGISTRATION for the seminar is an essential requirement for those who wish to request the CERTIFICATE OF PARTICIPATION, which, together with the signature on the ATTENDANCE SHEET, will confirm actual participation.