- Role: Ricercatore
- Assignments:Responsabile Orientamento Biotecnologie
- Office:U4 stanza 4016
- Telephone number:02 6448 3349/3451
- Reception Hours:su appuntamento
Microbial cells are used to obtain several products and metabolites of wide interest, such as organic acids, enzymes and hormones. The physiology and the metabolic routes of the producing host cells are deeply studied, coupling molecular biology, transcriptomic and metabolomic techniques with chemostat studies and flow cytometric single cells analyses. Productions in flasks are then optimised in fermentators, in order to increase yield and to minimise the duration and costs of the whole processes. The same multidisciplinary approach is used to minimise the stress induced in the host cells by the production process itself and to understand the principle of cellular stress answer in general.
Partecipants: Brambilla Luca Giuseppe , Branduardi Paola , Frascotti Gianni , Porro DaniloOUTER MEMBRANE BIOGENESIS IN ESCHERICHIA COLI
Lipopolysaccharide (LPS) is a component of the Outer Membrane (OM) an essential cellular structure shared by all of Gram-negative bacteria. LPS is a major virulence determinant relevant to initial bacterial attachment, evasion of host defenses and establishment of infection. Little is known about the molecular details of OM biogenesis and in particular about the LPS transport to the cell surface. By using specific conditional lethal mutants of E. coli we are dissecting the LPS transport pathway to the cell surface to shed light on a fundamental process for bacterial cell life and to allow a better understanding of the mechanisms that control bacteria-host interactions. From these studies we also identified new targets, which will be used to develop novel antibacterials inhibiting the LPS biogenetic pathway.
Partecipants: Polissi Alessandra