- Role: Professore Associato
- Office:U3- III piano stanza 3045
- Telephone number:02- 64483337
GENETICS AND MOLECULAR BIOLOGY OF CELL DIFFERENTIATION
- MECHANISMS OF POST-TRANSCRIPTIONAL REGULATION OF MAMMALIAN GENE EXPRESSION AND THEIR ROLE IN HUMAN DISEASE
- MOLECULAR GENETICS OF DEVELOPMENT AND CELL DIFFERENTIATION IN MOUSE AND MAN
MECHANISMS OF POST-TRANSCRIPTIONAL REGULATION OF MAMMALIAN GENE EXPRESSION AND THEIR ROLE IN HUMAN DISEASE
Our Laboratory is interested in decoding the mechanisms involved in biogenesis of messenger RNA (mRNA) and in contributing to a better understanding of the pathogenesis of diseases caused by deregulation of this process. Eukaryotic messenger RNA precursors (pre-mRNAs) are synthesized and processed in the nucleus prior to their export to the cytoplasm, where they serve as templates for protein synthesis. Transcription is coupled spatially and temporally to capping of the pre-mRNA at the 5’ end, to splicing of introns and to 3’ end polyadenylation. Very little is known about how these events are spatially and temporally coordinated within the context of the nucleus to allow for the regulation of accurate gene expression.
Partecipants: Barabino Silvia Maria Luisa
MOLECULAR GENETICS OF DEVELOPMENT AND CELL DIFFERENTIATION IN MOUSE AND MAN
The development of complex organs and tissues, such as brain and the hematopoietic system, requires the ordered expression of key transcription factors controlling cell type- and tissue-specific gene expression. Stem cells represent the self renewing compartment of rapidly replicating cell types, as in the hematopoietic system, but are present, in small numbers, also in adult brain, heart and other organs which do not show active cell replication in adults.
The group uses a common set of approaches (conditional and standard targeted mutagenesis in mouse, cell culture and gene transduction, chromatin studies, etc.) to investigate the role of key transcription factors in the development, maintenance and differentiation of a variety of stem cells.
Partecipants: Nicolis Silvia , Ottolenghi Sergio , Ronchi Antonella