- Role: Professore Ordinario
- Office:U4- V piano- stanza 5012
- Telephone number:02- 64483553
Dettagli Progetti di RicercaDENDRITIC CELLS AND NATURAL KILLER CELLS
Natural Killer (NK) cells exert a direct anti-tumor and anti-microbial effect and can influence the development of adaptive T cell responses. Activation of NK cells is regulated by accessory cells such as dendritic cells (DC). Following activation, NK cells accumulate at the lymph nodes draining the site of infection, the key place in which DC and NK cell interactions occur. Taking advantage of the two-photon intravital microscopy technology the capacity of activated NK cells to reach the draining lymph nodes is investigated together with the DC-derived signals necessary for NK cell priming in inflammatory conditions induced by lipolysaccharides.
Partecipants: , Granucci Francesca
DENDRITIC CELLS AND REGULATION OF IMMUNE TOLERANCE
The immune system of vertebrate animals has the capacity to respond to perturbations (invading pathogens, stress signals) limiting self-tissue damage. Tolerance to tissue antigens is achieved through a combination of thymic and peripheral events that eliminate or inactivate potentially dangerous T cells. Several mechanisms have been proposed to explain the induction of tolerance in peripheral autoreactive T cells. Taking advantage of different transgenic and knock out mouse models the mechanisms through which dendritic cells induce T cell tolerance in peripheral lymphoid organs are investigated.
Partecipants: Granucci Francesca
DENDRITIC CELLS BIOLOGY AND IMMUNOGENOMICS
Development of innate and adaptive immune response during the course of a microbial infection is dependent upon early interactions between incoming microrganisms with immature dendritic cells (iDCs) which are the first immune cells interacting with the microbial agents. The recent improvements of sequencing technologies, and in particular the publication of the initial version of the human and mouse genome sequences, have opened the field of large-scale functional approaches of biological systems. We employ high-throughput technologies to investigate fundamental aspects of the immune system and their roles in health and disease. In order to identify key cellular genes involved in these processes, we use a transcriptomic approach in which modifications of cellular transcriptome are analysed at several times post-infection.
Partecipants: , Foti Maria
Francesca Granucci has pioneered systems biology approaches to study complex dynamic processes, such as host-pathogen interactions, the process of dendritic cell maturation and the role of dendritic cells in activating and controlling anti-tumor NK cell functions. More recently she focused her research activity on signalling events downstream of CD14/TLR4 within cells of the mammalian innate immune system and she identified some of the key functions played by the NFATc family of transcription factors activated in dendritic cells in response to PRR agonists.
– Dr. Ivan Zanoni, Dipartimento di Biotecnologie e Bioscienze, UNIMIB.
– Prof. Davide Prosperi, Dipartimento di Biotecnologie e Bioscienze, UNIMIB.
last update June 2017